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. 2020 Oct 7;40(41):7902–7920. doi: 10.1523/JNEUROSCI.1124-20.2020

Figure 2.

Figure 2.

Targeting ChR2 to retinotopically defined areas of visual cortex. A, Pseudo-colored intrinsic autofluorescence responses to visual stimuli presented in two locations in a PV-Cre mouse. Magenta and green features represent 2D-Gaussian fits of responses to stimuli at visual field locations depicted in the inset (magenta: 0° azimuth, −20° elevation; green: 25° azimuth, 20° elevation; Gabor SD = 10°). Dashed lines indicate horizontal and vertical meridians. A, Anterior; M, medial. B, ChR2-tdTomato fluorescence (2D-Gaussian fit) from the same cortical region shown in A. Area of LED illumination (2D-Gaussian fit) through the optic fiber positioned above ChR2-expressing V1. The retinotopic location corresponding to maximal expression was used in all behavioral sessions (shown in inset; 25° azimuth, 0° elevation; Gabor SD = 6.75°). C, Representative confocal image of ChR2-tdTomato expression in the visual cortex of a different PV-Cre mouse. D, Trial schematic of the contrast change detection task. A contrast change of each direction (increase or decrease) was selected for stimulation (±15% contrast change for all mice).