Fig. 3.

NaPB, but not genistein, restores the plasma membrane expression of MCT8^L291R in JEG1 and COS7 cells. a, b Twenty micrograms of whole cell homogenates (upper panels) and 30 µL of biotinylated protein (lower panels) from JEG1 cells (a) and COS7 cells (b) were analyzed by Western blotting. MCT8 was detected by anti-HA antibody. Beta-actin served as loading control. c, d Quantification of three Western blot analyses obtained from overexpressing JEG1 cells (c) and overexpressing COS7 cells (d). NaPB (upper panels) rescued the plasma membrane expression of MCT8^L291R while genistein (lower panels) did not show any positive effect. Values are given as ±SD. The signal for ACTIN/actin was used for normalization. Statistical analyses: Two-way ANOVA with Dunnett's correction for multiple comparisons. Comparison of untreated/DMSO-treated MCT8^WT (darkest bars) with every other sample. * p < 0.05, ** p < 0.01. DMSO, dimethyl sulfoxide; HA, hemagglutinin; MCT8, monocarboxylate transporter 8; NaPB, sodium phenylbutyrate; SD, standard deviation.