Fig. 1.

Activity in vitro of PM against promastigotes (A) and intracellular amastigotes (B-D) of L. amazonensis M2269 strain and ER256 isolate. (A) Promastigotes were growth in increasing concentrations of PM and viability of the parasites was determined by the MTT assay after 24 h. (B) BMDM were infected with stationary-phase promastigotes and exposed to increasing concentrations of PM for 72 h. The percentage of infection was determined by counting 100 macrophages per coverslip. (C) PM susceptibility of intracellular amastigotes. Each bar represents the percentage of infected BMDM in different concentrations of PM (1–200 μM). The average ± standard deviation of three independent experiments is shown. (D) Number of amastigotes per infected macrophage treated with different concentrations of PM.