Fig. 3.

Histological analysis of the infected mice with L. amazonensis M2269 or ER256 isolate. Animals were euthanized at the end of the treatment with PM (8th week post-infection) and then infected hind footpad fragments were isolated, washed with PBS, fixed with formalin and processed with paraffin. Sections were stained with haematoxylin-eosin and then visualized in a light microscope. Images of untreated and treated animals with 150 and 600 mg/kg PM infected with L. amazonensis M2269 strain (A, B and C respectively) or L. amazonensis ER256 clinical isolate (D, E and F respectively). Arrows in the Figure indicate amastigotes inside parasitophorous vacuoles. Bar: 9 μm.