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. 2020 Sep 15;21(11):1502–1512. doi: 10.1111/mpp.12987

FIGURE 1.

FIGURE 1

The pipelines of PacBio and cDNA pathogen‐enrichment sequencing (PenSeq). (a) The pipeline of PacBio gDNA PenSeq. Briefly, the gDNA isolated from various Phytophthora infestans was enriched for RXLR effectors, sequenced by PacBio and de novo assembled for data mining. (b) The pipeline of cDNA PenSeq. The cDNA was synthetized using RNA sampled from various P. infestans at different stages (mycelium, zoospore, 12 hr postinoculation, 1, 2, and 3 days postinoculation). The libraries enriched for RXLR effectors were sequenced, reads were mapped to the RXLRome of the reference P. infestans genome T30‐4 and the expression levels of samples were calculated and compared. Black lines with dots represent the baits, the enriched fragments are depicted in blue (EU_13_A2), yellow (EC1_A1), pink (EU_6_A1), and green (US23). The black boxes indicate RXLR effectors. EU_13_A2, EC1_A1, EU_6_A1 and US23 are P. infestans isolates.