FIGURE 2.

Transcriptome analysis of NbRPL10A‐silenced Nicotiana benthamiana plants treated with nonhost pathogen. Two‐week‐old N. benthamiana plants were inoculated with TRV::GFP (vector control‐VC, UI‐ uninfected) or TRV::NbRPL0A constructs. Three weeks after TRV inoculation, the plants were vacuum‐infiltrated with nonhost pathogen Pseudomonas syringae pv. tomato T1 at 10⁴ cfu/ml concentration. Leaf samples were collected 12 hr after pathogen inoculation, RNA was isolated and subjected to RNA‐Seq experiment. (a) Venn diagram showing up‐ and down‐regulated genes in NbRPL10A‐silenced plants over vector control uninfected (VC UI) samples and 12 hr after pathogen treatment. (b) Venn diagram showing up‐ and down‐regulation of genes in NbRPL10A‐silenced or VC plants 12 hr after pathogen treatment compared to uninfected. (c) MapMan analysis of differentially regulated genes in uninfected NbRPL10A silenced plants compared to uninfected VC. (d) Differential expression of genes in NbRPL10A‐silenced plants compared to VC 12 hr after nonhost pathogen inoculation