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. 2020 Aug 6;295(41):14040–14052. doi: 10.1074/jbc.RA120.015036

Figure 2.

Figure 2.

Pyroptosis deficiency leads to increased cell death after coronavirus infection. A, real-time analysis of cell death in BMDMs using the IncuCyte imaging system and SYTOX Green nucleic acid staining after infection with MHV. Quantification (left) and images (right) of the cell death at the indicated time points are shown. The red in cell death images denotes the dead cells counted during the analysis. Scale bar, 50 µm. B and C, real-time analysis of cell death in BMDMs using the IncuCyte imaging system and SYTOX Green nucleic acid staining after infection with IAV (B) or VSV (C). D, immunoblot analysis of pro- (p45) and cleaved CASP1 (p20), pro- (p53), activated (p30), and inactivated (p20) GSDMD, pro- (p55) and cleaved CASP8 (p18), pro- (p35) and cleaved CASP7 (p19), pro- (p35) and cleaved CASP3 (p17), and pMLKL in BMDMs after MHV infection for 12 h. GAPDH was used as the internal control. Data are shown as mean ± S.E. (error bars) (A–C). *p < 0.05 and ****p < 0.0001 (one-way ANOVA). Data are representative of at least three independent experiments. ns, not significant; hpi, hours post-infection.