Figure 8.

The β-arr1–4A mutant attenuates CXCR4-promoted autophosphorylation of FAK in cells. a, HeLa cells transfected with empty vector (pCMV), FLAG-tagged β-arr1 WT, mutant (4A), or N domain fragment (residues 25–161) were stimulated with 30 nm CXCL12 for 15 min. Whole cell lysates were analyzed by immunoblotting for the indicated proteins. Representative immunoblots are shown. b and c, bars represent the average levels of pTyr³⁹⁷–FAK (pFAK) (b) or pERK-1/2 (c) relative to pCMV-transfected cells stimulated with CXCL12 from four independent experiments. The error bars represent the S.D. The data were analyzed by two-way analysis of variance followed by Bonferroni's multiple comparison test. The p values between indicated groups are shown (b), and the asterisks indicate p < 0.05 in each transfection condition.