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. 2020 Aug 14;295(41):14222–14235. doi: 10.1074/jbc.RA120.014228

Figure 1.

Figure 1.

NEIL3 Zf-GRF repeat interacts with APE1 and PCNA but not APE2 in vitro. A, GST pulldown assay performed between Myc-APE1 and GST-NEIL3-ZF1&2, GST-NEIL3-ZF1, or GST (4 μm) in vitro. B, interaction between Myc-APE2 and GST-NEIL3-ZF1&2, GST-NEIL3-ZF1, or GST (4 μm). C, interaction of GST-NEIL3-ZF1&2 to His-PCNA (4 μm) was detected, whereas GST-NEIL3-ZF1 had almost 50% reduction in interaction to His-PCNA. D, GST pulldown assay expanded to detect interaction between Myc-NEIL3-ZF1&2/Myc-NEIL3-ZF1/Myc-NEIL3-ZF2 and GST-APE1 (4 μm) in vitro. E, Myc-APE1 WT, E95Q, E95Q-D209N, or D306A interactions with GST-NEIL3-ZF1&2 (4 μm) for determining the possible residue within APE1 for such interaction. * indicates degradation products. IB, immunoblot.