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NEIL3 Zf-GRF repeat binds to shorter ssDNA than NEIL3-ZF1. A, interaction between GST-NEIL3-ZF1&2 (4 μm) and biotin-labeled ssDNA structures with lengths of 10, 20, 40, or 60 nt (5 nm) via biotin-DNA binding assays. B, the interaction of GST-NEIL3-ZF1 and different biotin-labeled ssDNA structures of 20, 40, or 60 nt. C, interaction of GST-NEIL3-ZF1&2 K553A and biotin-ssDNA structure with different lengths via biotin-DNA binding assays. IB, immunoblot.
