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. 2020 Aug 14;295(41):14222–14235. doi: 10.1074/jbc.RA120.014228

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© 2020 Ha et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 3. — NEIL3 Zf-GRF motifs interact with different ssDNA structures. A–C, EMSA show the interaction between ssDNA39 and GST-NEIL3-ZF1&2, GST-NEIL3-ZF1, or GST-NEIL3-ZF1&2 K553A at different doses as indicated at room temperature for 60 min. D-F, EMSA reveal interaction between ssDNA39-AP and increased concentrations of GST-NEIL3-ZF1&2 or GST-NEIL3-ZF1 or GST-NEIL3-ZF1&2 K553A in vitro. G-I, interaction between ssDNA70 and GST-NEIL3-ZF1&2 or GST-NEIL3-ZF1 but not with GST-NEIL3-ZF1&2 K553A via EMSA.