Figure 6.

Overexpression of the NEIL3 Zf-GRF repeat reduces the generation of DNA damage in oxidative stress in Xenopus egg extracts. A and C, GST-NEIL3-ZF1&2 (designated as ZF1&2) or GST (100 ng/µg) were added to Xenopus egg extracts supplemented with sperm chromatin DNA and hydrogen peroxide. After different incubation times (i.e. 0, 30, or 60 min), COMET assays under neutral condition (A) or alkaline condition (C) were performed. Representative images were shown. Scale bar in A and C represents 1 μm. B and D, Tail moment of images from COMET assays was quantified with the COMET Assay IV software (B for A and D for C, respectively) and statistical analysis was performed with GraphPad Prism8 (unpaired t test). n.s. indicates no significance (p > 0.05). ****, indicates p < 0.0001. n = 50, 56, 57, 67, 60, and 71, respectively, in B. n = 56, 55, 56, 57, 56, and 61, respectively, in D.