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. 2020 Jun 29;295(41):14125–14139. doi: 10.1074/jbc.RA119.010946

Figure 4.

Figure 4.

miR-378 reduces neuron autophagy by down-regulating GRB2. A, neuron autophagy detected after MDC staining (400×). B, neuronal death rate measured by LDH leakage rate. C, the protein levels of LC3-II/(LC3-I + LC3-II) and Beclin I normalized to β-actin in MCAO-operated mice (3 days post-MCAO) by Western blotting analysis. D, the protein levels of LC3-II/(LC3-I + LC3-II) and Beclin I normalized to β-actin in OGD-exposed neurons by Western blotting analysis; measurement data are expressed as means ± standard deviation, and comparison among multiple groups was analyzed by one-way analysis of variance with Tukey's post hoc test. *, p < 0.05 versus the control group (untreated neurons); #, p < 0.05 versus the OGD group (OGD-exposed neurons); &, p < 0.05 versus the miR-378 inhibitor OGD group (neurons treated with miR-378 inhibitor and exposed to OGD); ∼, p < 0.05 versus the GRB2 siRNA OGD group (neurons treated with GRB2 siRNA and exposed to OGD).