Figure 3.

Human and yeast RPA promote R-loop formation. A, human RPA (HsRPA) (200 nm) and yeast RPA (ScRPA) (100 nm), but not E. coli SSB (EcSSB) (270 nm), promote R-loop formation between a 48-mer RNA (no. 501; 3 μm, nt) and pUC19 dsDNA (67.2 μm, nt). The R-loops were analyzed by electrophoresis in a 1% agarose gel. B, data from A presented as a graph. C, kinetics of R-loop formation by ScRPA (100 nm) between 48-mer RNA (no. 501; 3 μm, nt) and pUC19 dsDNA (67.2 μm, nt) analyzed by electrophoresis in a 1% agarose gel. D, data from C presented as a graph. Error bars, S.E.