Figure 1.
A Tripartite Spatiotemporal Gene Expression Control System based on Dual Knocking Out Two Transcriptional Terminators
(A) Design and working principle of the tripartite FLP/CRE-out-mediated gene expression control system. The system consists of three vectors: a core vector and two plasmids encoding CRE and FLP recombinases. The core plasmid consists of sequences of a strong pan-expression eft-3 or a promoter of the hsp-16 family, two transcription terminators flanked by loxP or FRT recombination sites, a target gene and lin-44p::GFP.
(B) Confocal fluorescence images of a ZXW1988 transgenic worm. Different fluorescent channels show expression profile of green, srb-6p in ASHs and ADLs; blue, sra-6p in ASHs and ASIs; and red channels, TagRFP-T in ASHs. The merged image (right) indicated the co-localization of the three fluorescence colors. Scale bar, 20 μm.