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. 2020 Sep 15;23(10):101567. doi: 10.1016/j.isci.2020.101567

Figure 1.

Figure 1

A Tripartite Spatiotemporal Gene Expression Control System based on Dual Knocking Out Two Transcriptional Terminators

(A) Design and working principle of the tripartite FLP/CRE-out-mediated gene expression control system. The system consists of three vectors: a core vector and two plasmids encoding CRE and FLP recombinases. The core plasmid consists of sequences of a strong pan-expression eft-3 or a promoter of the hsp-16 family, two transcription terminators flanked by loxP or FRT recombination sites, a target gene and lin-44p::GFP.

(B) Confocal fluorescence images of a ZXW1988 transgenic worm. Different fluorescent channels show expression profile of green, srb-6p in ASHs and ADLs; blue, sra-6p in ASHs and ASIs; and red channels, TagRFP-T in ASHs. The merged image (right) indicated the co-localization of the three fluorescence colors. Scale bar, 20 μm.