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. 2019 Oct 22;11(10):1855–1862. doi: 10.1021/acsmedchemlett.9b00372

Figure 3.

Figure 3

FC-11 induced degradation of FAK via ubiquitin-proteasome system. (a) Chemical structure of FC-11. (b) FAK degradation at the indicated dose of FC-11. (c) Quantitative analysis of FAK levels after FC-11 treatment. The cells were treated by FC-11 at the indicated doses for 8 h in PA1 cells, and the data was collected from three independent experiments. (d) Confirmation of CRBN-based mechanism in driving degradation of FAK upon FC-11 treatment. PA1 cells were treated for 8 h with FC-11 (+, 1 nM; ++, 10 nM; +++ 100 nM) alone, or pomalidomide (10 μM) or PF562271 (10 μM) alone, or combination of FC-11 with pomalidomide or with PF562271. (e) Confirmation of proteasome-based mechanism in driving degradation of FAK upon FC-11 treatment. PA1 cells were treated for 8 h with FC-11 (+, 1 nM; ++, 10 nM; +++ 100 nM) alone, MG132 (5 μM) or carfilzomib (5 μM) alone, or a combination of FC-11 with MG132 or with carfizomib.