CypA Inhibition Modulates Phosphorylation of PRLr/Jak2 Signaling Intermediates
(A) T47D cells were serum starved for 16–24 h, pre-treated with NIM811 (10 μg/mL) or DMSO (vehicle control) for 4–6 h, and/or stimulated with human PRL (250 ng/mL) for the indicated times. Blots were probed with the indicated antibodies.
(B) Quantification of fold expression of phospho-proteins normalized to total proteins in (A) as indicated. Lines, mean of three independent experiments; error bars, ±SEM. ∗∗p < 0.01, ∗∗∗p < 0.005, ∗∗∗∗p < 0.001. Unpaired two-tailed t test was performed to determine significance.