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. 2020 Sep 19;23(10):101581. doi: 10.1016/j.isci.2020.101581

Figure 2.

Figure 2

Silencing of CypA Modulates Phosphorylation of PRLr/Jak2 Signaling Intermediates and Can Be Rescued by CypA-WT

(A) Stable expression of non-silencing control or CypA shRNA in T47D cells. Cells were serum starved for 16–24 h and stimulated with PRL (250 ng/mL) for 15 min. Immunoblots were probed with the indicated antibodies.

(B) Quantification of fold expression of phospho-proteins normalized to total proteins in (A) as indicated. Columns, mean of at least three independent experiments: error bars, ±SEM. ∗∗p < 0.01, ∗∗∗p < 0.005. Unpaired two-tailed t test was performed to determine significance.

(C) Control or CypA shRNA and/or CypA-WT (wild-type) were transfected and treated similarly as in (A).

(D) Quantification of fold expression of phospho-proteins normalized to total proteins in (C) as indicated. Columns, mean of three independent experiments: error bars, ±SEM. ∗∗p < 0.01, ∗∗∗p < 0.005. Unpaired two-tailed t test was performed to determine significance.