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. 2020 Sep 19;23(10):101581. doi: 10.1016/j.isci.2020.101581

Figure 3.

Figure 3

mRNA and Protein Expression of Prolactin-Responsive Genes, CISH and cyclin D1

(A) mRNA expression of CISH and cyclin D1. T47D cells pre-treated with NIM811 (10 μg/mL) for 4 h followed by PRL (250 ng/mL) stimulation for 2 h. Columns, mean of three independent experiments; error bars, SEM. ∗p < 0.05, ∗∗∗p < 0.005. Unpaired two-tailed t test was performed to determine significance. (B) Protein expression of CISH and cyclin D1 in T47D cells treated the same way as in (A). Quantification of % expression of CISH (C) and cyclin D1 (D) normalized to GAPDH in (B). Columns, mean of three independent experiments; error bars, SEM. ∗∗p < 0.01. Unpaired two-tailed t test was performed to determine significance.