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. 2020 Sep 19;23(10):101581. doi: 10.1016/j.isci.2020.101581

Figure 5.

Figure 5

NIM811 Treatment of T47D and MDA231 Cells Markedly Decreased Cell Viability and Anchorage-Independent Growth

(A) T47D and MDA-MB-231 cells were incubated with various doses of NIM811. Viable cells were quantified every other day by trypan blue exclusion. Lines, mean of three independent experiments; error bars, ±SEM. ∗∗p < 0.01, ∗∗∗p < 0.005. One-way ANOVA with Bonferroni's test to the control was used to determine significance.

(B) T47D and MDA-MB-231 cells incubated with or without various does of NIM811 were mixed with 0.45% agarose and overlaid over 0.8% bottom agar in 6-well plates. Cells were incubated for 14–21 days. Five randomly selected fields were counted for each well to assess anchorage-independent growth. Column, mean of three independent experiments; error bars, ±SEM. ∗∗p < 0.01, ∗∗∗p < 0.005. One-way ANOVA with Bonferroni's test to the control was used to determine significance.