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. 2020 Sep 19;23(10):101581. doi: 10.1016/j.isci.2020.101581

Figure 6.

Figure 6

CypA Inhibition Significantly Reduced TNBC Cell Migration

(A) Wound-healing assay. Confluent MDA-MB-231 cells were wounded with a pipette tip and cultured in serum-free medium in the presence of various doses of NIM811. Representative images of wound closure assay were acquired with a phase-contrast microscope, and the percentage of the wound closed was quantified. Scale bar, 100 μm. Columns, mean of three independent experiments; error bars, ±SEM. ∗∗p < 0.01. One-way ANOVA with Dunnett's test was used to determine significance.

(B) Migration was measured by a modified Boyden chamber assay. PRL-stimulated cell migration in T47D and MDA-231 cells was inhibited by NIM811. SFM (serum-free media) was the negative control. Columns, mean of three independent experiments; error bars, ±SEM. ∗p < 0.05, ∗∗p < 0.01. One-way ANOVA with Dunnett's test was used to determine significance.