Table 1.

Summary of Predicted Peroxisomal Proteins Identified in Three Genera of Pathogenic Free-Living Amoebae

Protein	Amoebozoa: Discosea, Centramoebia, Acanthopodida				Excavates: Discoba, Heterolobosea, Vahlkampfiidae
	Acanthamoeba castellanii	Acanthamoeba polyphaga	Acanthamoeba royreba	Balamuthia mandrillaris	Naegleria gruberi	Naegleria fowleri	Naegleria lovaniensis
PEX1	+	+	+	+	+	+	+
PEX2	+	+	+	+	+	+	+
PEX3	+	+	+	+	+	+	+
PEX4	+	+	+	+	+	+	+
PEX5	+	+	+	+	+	+	+
PEX6	+	+	+	+	+	+	+
PEX7	+	+	+	+	+	+	+
PEX10	+	+	+	+	+	+	+
PEX11a	+	+	+	+	+	+	+
PEX11b	+	+	+	+	+	+	+
PEX11c	+	+	+	nf	nf	nf	nf
PEX12	+	+	+	+	+	+	+
PEX13	+	+	+	+	nf	nf	nf
PEX14	+	+	+	+	nf	nf	nf
PEX16	+	+	+	+	+	+	+
PEX19	+	+	+	+	+	+	+
PEX22	+	+	+	+	+	+	+
PEX26	+	+	cn	cn	nf	nf	nf
Catalase	+, PTS1	+, PTS1	+, PTS1	+, PTS1	+	+, PTS1	+, PTS1

Note.—The presence (+) or absence (nf, not found) of the different putative peroxins identified in the Amoebozoans and Heteroloboseans included in our study is shown. Predicted sequences whose identity needs to be confirmed are specified (cn, confirmation needed). The different members of the Pex11 family found in these amoebae are labeled as Pex11a, Pex11b, and Pex11c. The presence (+) of at least one putative catalase sequence is also indicated. PTS1, peroxisomal targeting signal 1. Details regarding taxonomic classification can be found in Adl et al. (2019).