Exposure to maternal obesity activates the hypothalamic Notch signalling pathway and reduces neuronal markers. A) Quantitative real-time PCR analysis of Notch1, Hes1, Hes5, Ascl1 and Ngn2 in neonatal hypothalamic tissue (A.U., expressed as relative to Off-C, n = 5–6) B) Quantitative real-time PCR analysis of NeuN and Gfap in the neonatal hypothalamic tissue (A.U., expressed as relative to Off-C, n = 5–6). Off-C = white bars, Off-Ob = black bars. Data are presented as mean +/− SEM. ∗p < 0.05,∗∗∗p < 0.001.