Table 3.
Supplier details and dilutions for primary and secondary antibodies used in Western blotting experiments.
Antibody name | Supplier | Dilution |
---|---|---|
INSR | Santa Cruz (SC-711) | 1:200 |
IRS1 | Millipore (06–248) | 1:1,000 |
P- IRS1 (SER 307) | Upstate Biotech (07–247) | 1:1,000 |
AKT | Cell Signalling (2967) | 1:1,000 |
P- AKT (THR 308) | Cell Signalling (9275) | 1:1,000 |
ERK1/2 | Cell Signalling (9102) | 1:1,000 |
P- ERK1/2 | Cell Signalling (9101) | 1:1,000 |
Anti-rabbit-IgG-HRP | Abcam (AB6721) | 1:20,000 |
Anti-mouse-IgG-HRP | Abcam (AB6728) | 1:20,000 |