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. 2020 Oct 12;18:100. doi: 10.1186/s12958-020-00658-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effects of CsA on H₂O₂-induced expression of p-p53, p53, Bax and Bcl-2 in JEG-3 cells. a JEG-3 cells were pretreated with 1 μM CsA for 24 h followed by treatment with 500 μM H₂O₂ for another 24 h. Total cell lysates were harvested for western blot analysis of p-p53, p53, Bax and Bcl-2 expression. GAPDH was used as a loading control. b Quantitative analysis of p-p53 and p53 expression levels. c Quantitative analysis of Bax expression levels. d Quantitative analysis of Bcl-2 expression levels. The data presented are representative of three independent experiments. *p < 0.05, compared to the control; ^# p < 0.05, compared to the H₂O₂-treated group