Fig. 3.

The miR-21/RUNX1/YAP axis regulated the immunosuppressive ability of MDSCs in lung cancer. a the expression of miR-21 analyzed by RT-qPCR; b the expression of RUNX1 and YAP measured by western blot analysis; c, d, the proportion of MDSCs in peripheral blood and tumor tissues of mice detected by flow cytometry; e, f the proportion of Th and CTL in peripheral blood and tumor tissues measured by flow cytometry; g, h the inhibitory effect of MDSCs on Th and CTL in peripheral blood and tumor tissues of mice analyzed by T cell proliferation assay; i the expression of IL-10, TGF-β and GM-CSF were detected by ELISA; j the expression of ARG-1 and iNOS measured by RT-qPCR and western blot analysis. Measurement data were expressed as mean ± standard deviation. * p < 0.05 vs. cells co-treated with antagomir NC and sh-NC or antagomir NC and oe-NC, # p < 0.05 vs. cells co-treated with miR-21 antagomir and sh-NC or miR-21 antagomir and oe-NC. Measurement data among multiple groups were compared by one-way ANOVA with Tukey’s post hoc test. The experiment was repeated three times