Fig. 4.

The effect of miR-21/RUNX1/YAP axis on immunosuppressive ability of MDSCs in lung cancer in vitro. a the proportion of MDSCs in peripheral blood cells before and after magnetic bead sorting; b the cell cycle of MDSCs analyzed by a flow cytometer; c apoptosis map of MDSCs after 24 h treatment; d the expression of IL-6 and other effectors in MDSCs measured by ELISA; e the expression of ARG-1 and iNOS detected by RT-qPCR and western blot analysis. Measurement data were expressed as mean ± standard deviation. * p < 0.05 vs. cells co-treated with antagomir NC and sh-NC or antagomir NC and oe-NC, # p < 0.05 vs. cells co-treated with miR-21 antagomir and sh-NC or miR-21 antagomir and oe-NC. Measurement data among multiple groups were compared by ANOVA with Tukey’s post hoc test. The experiment was repeated three times