Fig. 5.

The effect of miR-21/RUNX1/YAP axis on the immunosuppressive ability of lung cancer xenografted tumor in vivo. a representative image of xenotransplantation tumor formation in nude mice; b tumor volume of mice; c tumor mass of mice; d the expression of ARG-1 and iNOS analyzed by RT-qPCR; e the expression of miR-21 detected by RT-qPCR; f the expression of RUNX1, YAP, ARG-1 and iNOS measured by western blot analysis. Measurement data were expressed as mean ± standard deviation. * p < 0.05 vs. cells co-treated with antagomir NC and sh-NC or antagomir NC and oe-NC, # p < 0.05 vs. cells co-treated with miR-21 antagomir and sh-NC or miR-21 antagomir and oe-NC. Measurement data among multiple groups were checked by ANOVA with Tukey’s post hoc test. Data comparison among multiple groups at different time points was conducted using repeated measurement ANOVA with Bonferroni’s post hoc test. The experiment was repeated three times