Table 1.
The primer sequences of RT-qPCR
| Gene | Primer sequences |
|---|---|
| miR-21 (hsa) | F: GGACTAGCTTATCAGACTG |
| R: CATCAGATG CGTTGCGTA | |
| miR-21 (mus) | F: GACATCGCATGGCTGTACCA |
| R: CCATGAGATTCAACAGTCAACATC | |
| RUNX1 | F: TGATGGCTGGCAATGATGAA |
| R: TGCGGTGGGTTTGTGAAGAC | |
| YAP | F: CGCTCTTCAACGCCGTCA |
| R: AGTACTGGCCTGTCGGGAGT | |
| ARG-1 | F: CTCCAAGCCAAAGTCCTTAGAG |
| R: AGGAGCTGTCATTAGGGACATC | |
| iNOS | F: CCAAGCCCTCACCTACTTCC |
| R: CTCTGAGGGCTGACACAAGG | |
| GAPDH | F: TGAAGCAGGCATCTGAGGG |
| R: CGAAGGTGGAAGAGTGGGAG | |
| U6 | F: CTCGCTTCGGCAGCACA |
| R: AACGCTTCACGAATTTGCGT |
RT-qPCR, reverse transcription quantitative polymerase chain reaction; RUNX1, runt-related transcription factor 1; YAP, yes-associated protein; ARG-1, Arginase-1; iNOS, inducible nitricoxide synthase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward ,R, reverse