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. 2020 Oct 11;8:146. doi: 10.1186/s40168-020-00921-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Ontogenetic shift of Triatoma rubida microbiomes in sylvatic populations from Arizona. Pictures of each T. rubida life stage are to proportional scale. a The microbiome profiles showing the 20 most abundant bacterial genera, listed in decreasing order of abundance to the right of the figure. Each column represents a single individual. LCBD, local contributions to beta diversity. The size of the circle for each sample indicates the degree of uniqueness for that sample relative to the overall variation in community composition [81]. b Richness and Shannon index (Y axis) for each developmental stage (X axis). Each point represents an individual bug. Length of the horizontal bar indicates the two stages being compared. Asterisks designate significance level as follows ***≤ 0.001, **≤ 0.01, *≤ 0.05. c NMDS on Bray-Curtis dissimilarity values for microbiomes of different developmental stage. Each colour and shape correspond to a different instar; L6 stands for adults. Each point represents an individual bug. Ellipses are statistically significant at 0.05 confidence interval