TABLE 2.
Correlation between fructose-responsive microbiota and host fructose signature genes in 3 metabolic tissues in B6 and DBA mice1
| Mouse strain and fructose-responsive microbiota | No. of correlated genes at FDR <0.05 | Correlation with host fructose-responsive genes | ||
|---|---|---|---|---|
| Host tissue | Top correlated genes2,3 | Overrepresented pathways3 | ||
| B6 (feces)4 | ||||
| Rikenellaceae | Hypo | 37 | Emg1, Rpl37a, Oat, Gna 13, Ctsz* | RNA metabolism, protein localization in endoplasmic reticulum, ribosome, amino acid metabolism, influenza infection |
| S24–7 | Hypo | 11 | Numbl*, Cxx1c*, Gnb2*,1500009L16Rik*, Slc25a17* | — |
| Dehalobacteriaceae | Hypo | 7 | Atp2b2*, Slc6a3*,Nefh*,Nrarp, Hint2 | — |
| Dehalobacterium | Hypo | 42 | Atp2b2*, Slc6a3*,Nefh*, Nrarp, Atg3* | Ribosome, response to alcohol, blood circulation, dopamine biosynthesis, locomotory behavior |
| Lachnospiraceae | Hypo | 2 | Maneal, Rbm39 | — |
| Mogibacteriaceae | Hypo | 54 | Mogs, Snrpe,Nrarp, Timp3*,Sgsm1 | Ribosome, metabolism of proteins, RNA, organonitrogen compound, protein targeting to membrane metabolism |
| Mogibacteriaceae (unknown genus) | Hypo | 130 | Mogs, Psmb4, Derl1, Timp3*,Sdcbp | Ribosome, metabolism of protein, RNA, protein localization to endoplasmic reticulum, signaling by robo receptors |
| Ruminococcaceae | Hypo | 12 | Trappc2l, Gnb2, Uck1, Mid1ip1, Itm2a* | — |
| Pseudomonadaceae | Hypo | 2 | Cxx1c*, Numbl* | — |
| Verrucomicrobiaceae | Hypo | 13 | Scarna2*, AW209491*,Gja1,Rps9*,Th | Ribosome, protein localization to endoplasmic reticulum, mRNA activation |
| Akkermansia | Hypo | 44 | Gja1,Rps9*,Th, Bmp7, Oxt* | Ribosome, protein localization to endoplasmic reticulum, selenoamino acid metabolism, influenza infection, signaling by robo receptors |
| DBA (cecum)4 | ||||
| Erysipelotrichaceae (unknown genus) | Liver | 5 | Gsdmd*, Wdr82*, Tmem62*, S100a10*, Pgk1* | — |
| Clostridium | Liver | 5 | Epha2*, Mad2l2*, Cyp8b1*, Ero1l*, Pus10* | — |
| Anaerostipes | Liver | 4 | Rogdi*, Cyp8b1*, Mad2l2*, Baiap2* | — |
| DBA (feces)4 | ||||
| Rikenellaceae | Adipose | 447 | Ccr1, Sema3e, Msr 1, Abhd3, Fas | RNA metabolism, response to lipid, cytokines, bacterial molecule, lipid metabolism, immune system, endocytosis |
| Rikenellaceae (unknown genus) | Adipose | 432 | Ccr1, Apobec1, Sema3e, Creb1, Fas | RNA metabolism, response to lipid, cytokines, bacterial molecule, lipid metabolism, immune system, signaling pathway |
| Pseudomonadaceae | Adipose | 77 | Sfrp4*, Msr1*, Sema3e*, Abhd3*, Fas* | — |
| Pseudomonadaceae (unknown genus) | Adipose | 209 | Creb1*, Fabp3*, Msr1*, Sema3e*, Fas* | RNA processing, response to cytokine, lipid, defense response, lipid metabolism, response to hormone |
| Pseudomonas | Adipose | 43 | Ppp1r13b*, Jmjd1c*, Msr1*, Slc25a10*, Fabp3* | — |
1
Fructose-responsive genes in hypothalamus (Hypo), liver, and adipose tissue. B6, C57BL/6J; DBA, DBA/2J; FDR, false discovery rate. Correlations were done at both family or genus levels. The microbiota correlated at the genus level are italicized.
2
Top 5 correlated genes that belong to major gene sets analyzed using GSEA (Gene Set Enrichment Analysis). Genes with positive correlation with microbiota are indicated with an asterisk.
3
Full lists of correlated genes are shown in Supplemental Table 1 and enriched pathways are shown in Supplemental Table 2.
4
Sample size, n = 4–6/group/mouse strain.