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Bar graphs showing the signal intensity for fragment peaks ([M–SA–H]−, [M–CO2–H]−, [M–H2O–H]−) produced by GD1a and GD1b standards at low and high laser fluence. The signal intensity of the fragment peaks was normalized against the intact [M–H]− mass peak for the GD1 parent species. Values graphed are the mean of three mass spectra (five mass scans) and the error bars represent one standard deviation. SA, sialic acid
