FIG 3.
bamAA496P and bamAA499S mutations bypass the essentiality of BamD. (A) Immunoblot analysis of stationary-phase bamD null strains expressing bamAA496P or bamAA499S. RpoA was used as a loading control. (B) The indicated strains were normalized by OD600, serially diluted, and spotted onto medium containing vancomycin or bacitracin. Plates were grown overnight at 30°C. (C) Stationary-phase cultures were normalized by OD600, electrophoresed, and probed for the indicated proteins. RpoA served as a loading control.