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Multicopy suppressors of Era647-mediated cell filamentation. Shown are phase contrast-fluorescence images of TUC05 cells grown at 32°C to an OD600 of 0.1 and 0.2 and then induced to express era647 from the lambda pL promoter at 42°C for 3 h. The top left image shows TUC05 cells. The top right image shows TUC05 with plasmid pBR322. The other images show TUC05 with clones expressing the genes shown. Cells were also stained with DAPI to show DNA. Scale bar, 5 μm.
