FIG 5.

Overproduction of Era647 inhibits Z ring assembly. Cells containing pBAD30 derivatives of Era or Era647 were induced with l-arabinose (Ara) for 1 h in mid- to late logarithmic phase and then fixed and stained with DAPI (blue) and fluorescent lectin (red) and immunostained with anti-FtsZ (green). Arrows highlight examples of sharp Z rings at midcell, which were found in many of the cells expressing wild-type Era. A rare Z ring in the Era647-induced cells at the bottom. Scale bar, 5 μm.