FIG 8.
Cytotoxic activities of B. pertussis mutants. The results of LDH assays at an MOI of 125 (A) or 250 (B) are shown as a histograms. Error bars indicate the SEMs from triplicate experiments. *, P < 0.05. Experiments were performed at least three times, and representative data are shown. (C) MGKU1 (Tohama I nalidixic acid-resistant strain [1: WT Nalr]), MGKU2 (T3SS-inactive strain [2: ΔT3SS]), MGKU3 (BteA-deficient strain [3: ΔBteA]), or MGKU4 (BspR-deficient strain [4: ΔBspR]) was cultured in SS_1/20AsA_1/2CaA medium. WCL and Sup samples were separated by SDS-PAGE and analyzed by Western blotting with anti-BteA antibody. We loaded a 3-fold-smaller amount of MGKU4 supernatant fraction on the SDS-PAGE gel to avoid obtaining an excess signal intensity. NS, nonspecific signals. Loaded WCL and Sup samples were prepared from equal volumes of bacterial culture. Experiments were performed at least three times, and representative data are shown.