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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Clin Cancer Res. 2020 Apr 16;26(15):4093–4106. doi: 10.1158/1078-0432.CCR-19-4137

Figure 5: NF-κB, A20 and RBX1 regulate ABCB1 expression in HL lines.

Figure 5:

A. The L428 line was treated with indicated drugs at the following concentrations: MLN120B (20μM), BV (10μg/ml), or the combination for 24 hours. Surface ABCB1 expressions were measured by flow cytometry. The relative ABCB1 MFI was normalized to the untreated cells. B. KMH2, L428 and L540 lines were treated with MLN120B (20μM) for 24 hours. ABCB1 mRNA expressions were measured by real-time PCR and normalized to the untreated cells. C. HL cell line L428 was transduced with cDNA of IKKβ WT, IKKβ S177/181E or empty control, selected and expression induced. Surface ABCB1 expressions were measured by flow cytometry and normalized to the empty control. D and E, The L428 line was transduced with A20 or Ctrl sgRNAs, selected and expression induced. Relative ABCB1 expressions were measured by real-time PCR (D) and flow cytometry (E). F. The L428 line was transduced with A20 or Ctrl sgRNAs, selected and expression induced, then treated with the indicated concentrations of BV, ABCB1 inhibitor CsA, or both for 4 days. Viability was measured by MTS assay and normalized to PBS-treated cells. G. The HL cell line L428 was transduced with RBX1 or Ctrl sgRNAs, selected and expression induced, then treated with BV at the indicated concentrations for 4 days. Viability was measured by MTS assay and normalized to PBS-treated cells. H. The L428 line was transduced with RBX1 or Ctrl sgRNAs along with GFP. Surface CD30 expression in uninfected (GFP−) cells and sgRNA infected (GFP+) cells was measured by flow cytometry. The summary of three independent experiments is shown. The relative CD30 MFI was normalized to the uninfected (GFP−) cells. I. The L428 line was transduced with RBX1 or Ctrl sgRNAs, selected and expression induced, then treated with MMAE at the indicated concentrations for 4 days. Viability was measured by MTS assay and normalized to DMSO-treated cells. J and K. The L428 line was transduced with RBX1 or Ctrl sgRNAs, selected and expression induced. Relative ABCB1 expressions were measured by real-time PCR (J) and flow cytometry (K). L. The L428 line was transduced with RBX1 or Ctrl sgRNAs, selected and expression induced, then treated with the indicated concentrations of BV, ABCB1 inhibitor CsA, or both for 4 days. Viability was measured by MTS assay and normalized to PBS-treated cells. For all the panels, Error bars denote SEM of triplicates. * indicates P < 0.05; ** indicates P < 0.01; n.s indicates no statistical difference.