. 2020 Sep 28;14:571216. doi: 10.3389/fncel.2020.571216

TABLE 2.

Quantitative results and statistical analyses of volumetric analysis and mitochondria position in four zones are shown as Mean ± SD in striatum and TRN.

TRN

	Volumetric analysis

			1 month		3 months		6 months

			WT	KO	WT	KO	WT	KO

			Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Effect of age	→	p-value	Effect of genotype	→	p-value
		V_cyto	484.2 ± 55.3	582.8 ± 27.7	476.6 ± 52.4	590.2 ± 39.0	467.0 ± 34.4	572.4 ± 46.6	F(2,24) = 0.33040		0.7218	F(1,24) = 44.07		<0.0001
		V_mito	43.6 ± 11.3	115.8 ± 15.8	46.2 ± 13.5	114.2 ± 11.7	46.0 ± 12.8	117.6 ± 14.3	F(2,24) = 0.06775		0.9347	F(1,24) = 210.50		<0.0001
		V_nuclei	157.6 ± 34.0	187.2 ± 26.4	162.8 ± 28.2	174.2 ± 33.3	163.8 ± 36.2	183.6 ± 25.9	F(2,24) = 0.07659		0.0926	F(1,24) = 3.22		0.0853
		V_soma	641.8 ± 79.6	770.0 ± 39.4	639.4 ± 65.0	764.4 ± 34.6	630.8 ± 38.7	756.0 ± 39.0	F(2,24) = 0.14990		0.8616	F(1,24) = 43.89		<0.0001
		V_mito/V_cyto	9.1 ± 2.5	19.8 ± 1.9	9.7 ± 2.5	19.4 ± 2.8	9.8 ± 2.5	20.5 ± 1.9	F(2,24) = 0.26990		0.7658	F(1,24) = 144.10		<0.0001
		V_nuc/V_cyto	32.6 ± 5.7	32.2 ± 4.8	34.4 ± 6.2	29.79 ± 7.2	35.4 ± 9.5	32.4 ± 6.5	F(2,24) = 0.21200		0.8105	F(1,24) = 1.13		0.2972

			1 month		3 months		6 months
			WT vs. KO		WT vs. KO		WT vs. KO
			p-value		p-value		p-value

		V_cyto	0.0171*		0.0047**		0.0096**
		V_mito	<0.0001****		<0.0001****		<0.0001****
		V_nuclei	0.6593 ns		0.9913 ns		0.9092 ns
		V_soma	0.0081**		0.0102*		0.0101*
		V_mito/V_cyto	<0.0001****		<0.0001****		<0.0001****
		V_nuc/V_cyto	>0.9999 ns		0.8934 ns		0.9803 ns

	Mito volume density

			1 month		3 months		6 months

			WT	KO	WT	KO	WT	KO

			Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Effect of age	→	p-value	Effect of genotype	→	p-value

		Zone_I	10.1 ± 1.2	10.8 ± 0.8	9.8 ± 1.0	11.1 ± 1.5	9.4 ± 1.2	10.5 ± 1.3	F(2,24) = 0.54270		0.5881	F(1,24) = 5.76		0.0245
		Zone_II	43.5 ± 3.5	35.9 ± 3.1	44.8 ± 2.8	26.8 ± 3.3	45.1 ± 1.5	26.0 ± 3.0	F(2,24) = 6.27000		0.0064	F(1,24) = 191.50		<0.0001
		Zone_III	37.5 ± 3.2	39.2 ± 3.5	36.8 ± 2.6	47.9 ± 3.2	36.7 ± 2.1	49.2 ± 4.0	F(2,24) = 6.09300		0.0072	F(1,24) = 52.09		<0.0001
		Zone_IV	8.8 ± 1.1	14.2 ± 0.9	8.6 ± 1.2	14.2 ± 0.6	8.8 ± 1.3	14.2 ± 1.2	F(2,24) = 0.02404		0.9763	F(1,24) = 199.50		<0.0001

			1 month		3 months		6 months
			WT vs. KO		WT vs. KO		WT vs. KO
			p-value		p-value		p-value

		Zone_I	0.9340 ns		0.5091 ns		0.6882 ns
		Zone_II	0.0048**		<0.0001****		<0.0001****
		Zone_III	0.9639 ns		0.0002***		<0.0001****
		Zone_IV	<0.0001****		<0.0001****		<0.0001****

Volumetric analysis

			1 month		3 months		6 months

			WT	KO	WT	KO	WT	KO

			Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Effect of age	→	p-value	Effect of genotype	→	p-value

		V_cyto	769.0 ± 34.9	916.0 ± 78.5	777.6 ± 32.6	914.4 ± 85.4	784.8 ± 44.8	910.2 ± 59.4	F(2,24) = 0.01856		0.9816	F(1,24) = 39.33		<0.0001
		V_mito	48.8 ± 8.2	92.0 ± 7.3	47.2 ± 7.6	91.6 ± 7.9	46.4 ± 4.2	90.0 ± 6.9	F(2,24) = 0.23790		0.7901	F(1,24) = 281.3		<0.0001
		V_nuclei	335.0 ± 26.2	353.8 ± 24.3	333.8 ± 24.0	347.2 ± 15.1	342.6 ± 19.7	359.8 ± 23.7	F(2,24) = 0.58060		0.5672	F(1,24) = 4.027		0.0562
		V_soma	1104 ± 27.6	1270 ± 76.0	1111 ± 50.6	1262 ± 78.9	1127 ± 45.3	1270 ± 76.5	F(2,24) = 0.12400		0.8839	F(1,24) = 45.24		<0.0001
		V_mito/V_cyto	6.3 ± 0.9	10.1 ± 0.9	6.1 ± 1.0	10.04 ± 0.6	6.0 ± 0.7	9.9 ± 0.8	F(2,24) = 0.22840		0.7975	F(1,24) = 166.1		<0.0001
		V_nuc/V_cyto	43.7 ± 5.1	38.9 ± 5.2	42.9 ± 2.4	38.3 ± 4.8	43.8 ± 3.9	39.6 ± 2.2	F(2,24) = 0.16530		0.8486	F(1,24) = 8.981		0.0063

			1 month		3 months		6 months
			WT vs. KO		WT vs. KO		WT vs. KO
			p-value		p-value		p-value

		V_cyto	0.0078**		0.0148*		0.0297*
		V_mito	<0.0001****		<0.0001****		<0.0001****
		V_nuclei	0.7699 ns		0.9311 ns		0.8277 ns
		V_soma	0.0037**		0.0096**		0.0152*
		V_mito/V_cyto	<0.0001****		<0.0001****		<0.0001****
		V_nuc/V_cyto	0.4699 ns		0.5063 ns		0.6045 ns

	Mito volume density

			1 month		3 months		6 months

			WT	KO	WT	KO	WT	KO

			Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Effect of age	→	p-value	Effect of genotype	→	p-value

		Zone_I	9.0 ± 0.9	10.2 ± 1.6	8.6 ± 0.9	9.5 ± 1.0	9.1 ± 1.2	10.0 ± 1.5	F(2,24) = 0.64150		0.5353	F(1,24) = 5.166		0.0323
		Zone_II	40.6 ± 3.6	34.2 ± 1.6	41.9 ± 1.3	26.9 ± 1.8	41.1 ± 2.7	28.7 ± 2.1	F(2,24) = 4.66200		0.0195	F(1,24) = 177.3		<0.0001
		Zone_III	42.8 ± 4.6	42.8 ± 2.6	41.6 ± 2.2	51.2 ± 2.9	41.9 ± 2.8	48.5 ± 3.3	F(2,24) = 3.27200		0.0554	F(1,24) = 21.73		<0.0001
		Zone_IV	7.7 ± 0.8	12.8 ± 1.2	7.8 ± 0.9	12.5 ± 1.0	7.8 ± 0.8	12.7 ± 0.8	F(2,24) = 0.02351		0.9768	F(1,24) = 207.6		<0.0001

			1 month		3 months		6 months
			WT vs. KO		WT vs. KO		WT vs. KO
			p-value		p-value		p-value

		Zone_I	0.5666 ns		0.8726 ns		0.8498 ns
		Zone_II	0.0027**		<0.0001****		<0.0001****
		Zone_III	>0.9999 ns		0.0009***		0.0321*
		Zone_IV	<0.0001****		<0.0001****		<0.0001****

Two-way ANOVA was performed for each parameter followed by Tukey’s multiple comparison. The values were compared among groups using multivariate ANOVA followed by Tukey test for multiple comparisons. ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001.