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. 2020 Sep 25;57(5):1157–1168. doi: 10.3892/ijo.2020.5128

Figure 2.

Figure 2

LUNAR1 knockdown inhibits CRC cell proliferation and accelerates apoptosis. SW480 and LoVo cells were transfected with sh-LUNAR1 for 24 h to promote LUNAR1 knockdown, and sh-NC served as a negative control. (A) RT-qPCR was performed to detect LUNAR1 expression following transfection. (B) CCK-8 assay was performed to evaluate SW480 and LoVo cell viability. (C) Colony formation assay was conducted to assess SW480 and LoVo cell proliferation. (D) FACS analysis was performed to analyze cell cycle distribution. (E) Western blot analysis was performed to detect the levels of cell cycle-related proteins, including cyclin D1, cyclin E, p27 and p21. (F) Apoptosis analysis was performed to evaluate cell apoptosis. (G) Western blot analysis was conducted to measure the levels of apoptosis-related proteins, including Bax, Bcl2, cleaved-caspase 3 and cleaved-caspase 9. **P<0.01 vs. the sh-NC group. Comparisons were performed using paired t-test or one-way ANOVA. Error bars represent SD. Data represent three independent experiments. LUNAR1, lncRNA LUNAR1; CRC, colorectal cancer.