Figure 3.

LUNAR1 knockdown inhibits CRC cell migration and invasion. SW480 and LoVo cells were transfected with sh-LUNAR1 for 24 h to promote LUNAR1 knockdown, and sh-NC served as a negative control. (A) Wound-healing migration assay was conducted to assess cell migration. (B) Transwell chamber assay was performed to evaluate cell migration and invasion. (C) Western blot analysis was performed to detect the levels of invasion-related proteins, including Cox-2, MMP-2 and MMP-9. ^**P<0.01 vs. the sh-NC group. Comparisons were performed using paired t-test or one-way ANOVA. Error bars represent SD. Data represent three independent experiments. LUNAR1, lncRNA LUNAR1; CRC, colorectal cancer; Cox-2, cyclooxygenase 2; MMP-2, matrix metalloprotein 2; MMP-9, matrix metalloprotein 9.