Figure 2.

Deficiency of REST expression results in the upregulation of RE1‐containing genes. (a) Western blot analysis of REST expression in the KO and KD cells using anti‐REST polyclonal antibody. (b) The bar chart depicts the densitometric quantification of the signals obtained by Western blotting and the values show the relative REST band intensity relative to β‐actin. The expression of REST mRNA in the KO (c) and KD cells (d). The expression of SNAP25 (e), SYN1 (f), STMN2 (g), and GRIA2 (h) in the KO and KD cells. Data are the averages of 6 biological repeats normalized to the RNU6 housekeeping gene. The log2 expression values are represented by the circular dots, the p‐value was calculated by two‐tailed Student's t‐test, and the error bars depict SEM. EmpVec is the shRNA empty vector control