Figure 2.

A subset of Arabidopsis tRFs can inhibit efficiently translation in vitro. (A) Features of the 10 tRFs analysed in this work. Their sequences are presented in Supplemental Table 1 and the data derive from [28]. Besides, tRF-5A (Asp) was also found in AGO1 immunoprecipitates (L. Drouard, personal communication). (B) The synthetic tRFs presented in (A) were tested for their ability to inhibit the in vitro protein synthesis of pGFP. Experiments were performed essentially as described in Fig. 1C. Two concentrations of oligoribonucleotides (0.2 and 1 µM) were used. Quantifications of the results are presented in (C). An arbitrary value of 1 (dotted vertical line) has been given to in vitro synthesized pGFP in the absence of added tRF (Mock experiment: M). Error bars represent standard errors of the mean of independent biological experiments (n = 3 to 13). One-way ANOVA tests were used to calculate p-values. Asterisks indicate statistically significant differences between Mock and each treatment (**P< 0.01; *P< 0.1). Dark grey: 0.2 µM, pale grey: 1 µM.