Table 2.
Changes in localization and appearance of multiple seed sequence positions over time. Position-wise distribution is reported as a fraction of all the experimentally determined downregulated genes. Three main combinations of seed sequence localization (3ʹ-UTR only, 3ʹ-UTR and CDS, CDS only) are provided explicitly, while the ‘others’ graph contains the cases that are rarer: 5ʹ-UTR-only, 5ʹ-UTR and CDS, 3ʹ-UTR and 5ʹ-UTR and 3ʹ-UTR, CDS and 5ʹ-UTR.
| miRNA | Time point | Transcripts with multiple seed sequence positions |
# of unique seed sequences |
3ʹ-UTR-only | 3ʹ-UTR and CDS | CDS-only | Others | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 2 | 3 | 4 | ≥5 | ||||||||
| # | % of total | ||||||||||
| hsa-miR-17-5p | 12 h | 24 | 68.6 | 7 | 6 | 4 | 7 | 6 | 17 | 0 | 1 |
| 24 h | 23 | 56.1 | 9 | 6 | 2 | 6 | 3 | 15 | 2 | 3 | |
| 48 h | 20 | 46.5 | 7 | 6 | 2 | 5 | 3 | 9 | 4 | 4 | |
| hsa-miR-517a-3p | 12 h | 6 | 4.9 | 5 | 1 | 0 | 0 | 4 | 2 | 0 | 0 |
| 24 h | 13 | 3.8 | 12 | 1 | 0 | 0 | 9 | 3 | 1 | 0 | |
| 48 h | 1 | 0.1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | |
| hsa-miR-135b-5p | 12 h | 20 | 45.2 | 9 | 2 | 3 | 6 | 9 | 9 | 1 | 0 |
| 24 h | 44 | 45.3 | 10 | 9 | 8 | 17 | 12 | 23 | 2 | 2 | |
| 36 h | 30 | 16.1 | 19 | 6 | 4 | 1 | 9 | 16 | 5 | 0 | |