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. 2020 Jun 21;17(8):1092–1103. doi: 10.1080/15476286.2020.1779492

Figure 2.

Figure 2.

Impact of Alkbh1 knockdown on cell fate and tRNA cleavage after stress. A: Annexin V FACS analysis (left graph) and SYBR gold staining following Alkbh1 KD and arsenite stress (400 µM for 4 hours). Alkbh1 KD reduced the levels of apoptotic and necrotic cells and rescued tRNA from cleavage. B: Annexin V FACS analysis and SYBR gold staining following Alkbh1 KD and antimycin stress (150 µg/ml for 4 hours). Alkbh1 KD had no impact on cell fate or tRNA cleavage following antimycin stress. (Note: FACS for As and antimycin stresses were performed simultaneously but the results presented in 2 graphs for easier comprehension and data presentation. FACS data were presented as ratio to unstressed (control) cells). C: Annexin V FACS analysis and SYBR gold staining following Alkbh1 KD and OGD-R stress. Alkbh1 KD also had no impact on cell death ratio following OGD-R and no apparent impact on tRNA cleavage. Asterisk: p < 0.05, N.S: not significant statistically. SYBR gold staining was performed with 500ng total RNA per lane.