Figure 7.

Variable Nm modifications in T. brucei are located around functional domains of the ribosome. Nm sites whose levels are reproducibly regulated (>10%) in T. brucei BSF are indicated. Nm sites whose level is increased in BSF (compared to PCF) are coloured in red and those whose level is decreased in BSF are coloured in blue, respectively. Hypermodified and hypomodified sites (FC>3) are indicated by a green box. A- P- and E- site tRNAs are represented as a gold surface. The peptidyl transferase centre (PTC) and protein exit tunnel (marked with a purple dotted line) are indicated. The 3D representation is based on the published L. donovani cryo-EM coordinates deposited as 6AZ3 and 6AZ1 for the large and small subunits, respectively [21]. L.donovani Nm sites corresponding to T. brucei SSU and LSU rRNA are shown.