Table 1.

The complete stoichiometry of Nm sites in T. brucei rRNA. The identity and position of individual Nm sites in T. brucei rRNA. The RMS score of each individual Nm and its fold change are presented for both life stages. Data are shown as mean ± s.e.m. The fold change of 2ʹ-OMe score, identity and expression values of snoRNA guiding individual Nms are also presented.

rRNA	Nm	BSF (RMS)	PCF (RMS)	FC_RMS (BSF/PCF)	FC _2ʹ-OMe (BSF/PCF)	snoRNA	snoRNA log2FC(BSF/PC)
SSU	Um36	0.64 ± 0.02	0.83 ± 0.04	0.77	0.84	TB10Cs2C1	3.51
SSU	Cm46	0.52 ± 0.01	0.55 ± 0.01	0.95	0.94	TB8Cs3 C3	0.15
SSU	Am56	0.47 ± 0.03	0.75 ± 0.04	0.63	1.09	TB8Cs2C1	0.09
SSU	Cm66	0.35 ± 0.01	0.38 ± 0.06	0.93	1.10	TB8Cs2C1	0.09
SSU	Am125	0.83 ± 0.01	0.89 ± 0.00	0.94	0.98	TB8Cs1 C2	−0.58
SSU	Um680	0.52 ± 0.01	0.55 ± 0.01	0.94	1.19	TB10Cs3 C3	−0.60
SSU	Um714	0.78 ± 0.01	0.80 ± 0.05	0.98	1.34	TB9Cs2C6	1.41
SSU	Am721	0.76 ± 0.01	0.81 ± 0.06	0.95	1.46	TB6Cs2C1	−0.03
SSU	Gm1517	0.45 ± 0.01	0.48 ± 0.02	0.93	1.50	TB11 Cs3 C2	1.90
SSU	Gm1531	0.76 ± 0.01	0.84 ± 0.00	0.91	1.21	TB3Cs1C1	−0.66
SSU	Gm1603	0.89 ± 0.01	0.95 ± 0.00	0.93	0.88	TB8Cs3C2	1.35
SSU	Um1630	0.65 ± 0.03	0.04 ± 0.02	15.38	0.72	TB10Cs2”C3	1.03
SSU	Um1652	0.78 ± 0.01	0.72 ± 0.06	1.09	1.13	TB10Cs1C3	1.07
SSU	Um1674	0.23 ± 0.06	0.73 ± 0.02	0.31	0.95	TB10Cs3C2	−0.12
SSU	Gm1676	0.39 ± 0.08	0.14 ± 0.05	2.80	0.79	TB9Cs3C1	−0.50
SSU	Gm1700	0.64 ± 0.00	0.55 ± 0.02	1.18	0.92	TB10Cs2”C1	−0.07
SSU	Cm1844	0.42 ± 0.01	0.13 ± 0.07	3.12	1.25	TB7Cs1C1	1.01
SSU	Gm1895	0.52 ± 0.02	0.69 ± 0.04	0.75	1.48	TB9Cs2C4	−2.01
SSU	Um1899	0.76 ± 0.02	0.89 ± 0.03	0.86	1.20	TB11Cs2C1	−1.45
SSU	Um2054	0.93 ± 0.00	0.90 ± 0.02	1.03	0.85	TB8Cs2C0	0.33
SSU	Am2096	0.76 ± 0.01	0.87 ± 0.04	0.87	1.39	TB9Cs3C2	−0.01
SSU	Um2123	0.65 ± 0.01	0.57 ± 0.03	1.14	0.88	TB10Cs4C3	−0.04
SSU	Cm2134	0.72 ± 0.00	0.52 ± 0.01	1.38	1.29	TB10Cs4C3	−0.04
SSU	Um2154	0.10 ± 0.01	0.38 ± 0.14	0.25	0.85	TB10Cs3C2	−0.12
SSU	Gm2227	0.87 ± 0.01	0.92 ± 0.01	0.95	1.76	TB8Cs1C1	1.06
LSUβ	Gm71	0.61 ± 0.01	0.64 ± 0.03	0.94	1.17	TB9Cs1C1	−0.86
LSUβ	Um73	0.69 ± 0.01	0.57 ± 0.04	1.21	1.55	TB9Cs1C1	−0.86
LSUβ	Am95	0.74 ± 0.01	0.80 ± 0.02	0.92	1.16	TB3Cs3C1	1.06
LSUβ	Cm377	0.36 ± 0.02	0.31 ± 0.16	1.18	1.74	TB9Cs4C3	1.36
LSUβ	Am400	0.90 ± 0.00	0.92 ± 0.01	0.97	1.17	TB6Cs1C2	−1.58
LSUβ	Am520	0.73 ± 0.01	0.75 ± 0.05	0.98	0.57	TB10Cs7C2	1.61
LSUβ	Am544	0.07 ± 0.03	0.26 ± 0.26	0.27	0.55	TB6Cs1C3	0.01
LSUβ	Am545	0.75 ± 0.01	0.80 ± 0.03	0.94	0.55	TB6Cs1C3	0.01
LSUβ	Gm552	0.63 ± 0.02	0.74 ± 0.07	0.85	0.53	TB10Cs1C4	−3.04
LSUβ	Um578	0.90 ± 0.01	0.93 ± 0.01	0.97	1.19	TB11Cs3C1	0.88
LSUβ	Am588	0.41 ± 0.03	0.52 ± 0.09	0.79	1.04	TB8Cs1C3	0.57
LSUβ	Cm601	0.62 ± 0.01	0.33 ± 0.13	1.86	1.30	TB10Cs1C1	−0.72
LSUβ	Am609	0.50 ± 0.01	0.54 ± 0.03	0.94	1.25	TB10Cs1C1	−0.72
LSUβ	Am622	0.81 ± 0.01	0.81 ± 0.00	0.99	1.30	TB10Cs2ʹC1	0.47
LSUβ	Am646	0.62 ± 0.01	0.55 ± 0.08	1.12	1.23	TB11Cs4ʹC1	0.92
LSUβ	Gm659	0.12 ± 0.03	0.42 ± 0.12	0.29	1.13	TB9Cs2C7	−0.85
LSUβ	Um672	0.35 ± 0.01	0.31 ± 0.19	1.12	2.92	TB11Cs4C3	2.01
LSUβ	Gm673	0.60 ± 0.02	0.60 ± 0.02	1.01	2.60	TB11Cs4C3	2.01
LSUβ	Um685	0.62 ± 0.01	0.68 ± 0.08	0.91	1.40	TB9Cs2C7	−0.85
LSUβ	Um728	0.17 ± 0.02	0.36 ± 0.07	0.48	0.97	TB10Cs3C5	0.44
LSUβ	Um1093	0.68 ± 0.02	0.78 ± 0.01	0.87	1.16	TB5Cs1C1	−0.57
LSUβ	Gm1094	0.57 ± 0.02	0.70 ± 0.03	0.81	1.10	TB5Cs1C1	−0.57
LSUβ	Cm1175	0.22 ± 0.03	0.38 ± 0.08	0.58	1.43	TB5Cs1C1	−0.57
LSUβ	Am1201	0.77 ± 0.01	0.80 ± 0.04	0.97	1.67	TB10Cs2”C2	0.35
LSUβ	Gm1245	0.60 ± 0.01	0.53 ± 0.14	1.14	1.14	TB3Cs1C-1	1.31
LSUβ	Gm1247	0.72 ± 0.03	0.23 ± 0.13	3.07	1.02	TB11Cs1C2	0.66
LSUβ	Cm1264	0.81 ± 0.00	0.62 ± 0.04	1.31	0.94	TB6Cs1C1	1.62
LSUβ	Gm1269	0.37 ± 0.02	0.39 ± 0.16	0.96	0.69	TB11Cs1C2	0.66
LSUβ	Um1375	0.36 ± 0.04	0.63 ± 0.03	0.58	0.71	TB10Cs3C1	0.05
LSUβ	Am1388	0.29 ± 0.02	0.39 ± 0.16	0.75	0.86	TB11Cs4C1	0.72
LSUβ	Am1400	0.72 ± 0.01	0.71 ± 0.05	1.02	1.07	TB11Cs4C1	0.72
LSUβ	Cm1413	0.78 ± 0.01	0.74 ± 0.02	1.06	0.78	TB9Cs2C1	3.56
LSUβ	Um1435	0.60 ± 0.02	0.85 ± 0.05	0.71	0.95	TB9Cs3C3	0.20
LSUα	Am254	0.17 ± 0.03	0.38 ± 0.03	0.45	0.92	TB10Cs3C2	−0.12
LSUα	Am742	0.39 ± 0.01	0.85 ± 0.07	0.46	1.00	TB9Cs2C2	−0.75
LSUα	Am743	0.89 ± 0.00	0.95 ± 0.03	0.94	1.00	TB9Cs2C2	−0.75
LSUα	Am746	0.91 ± 0.00	0.94 ± 0.02	0.97	0.87	TB10Cs2C2	−0.70
LSUα	Cm747	0.61 ± 0.01	0.75 ± 0.02	0.80	0.85	TB10Cs2C2	−0.70
LSUα	Cm760	0.82 ± 0.01	0.85 ± 0.04	0.96	1.10	TB9Cs2C2	−0.75
LSUα	Um916	0.69 ± 0.01	0.63 ± 0.02	1.10	1.34	TB11Cs4C2	1.30
LSUα	Gm925	0.82 ± 0.01	0.87 ± 0.04	0.94	1.62	TB9Cs2C3	1.60
LSUα	Am927	0.69 ± 0.01	0.50 ± 0.17	1.39	1.35	TB11Cs4C2	1.30
LSUα	Am996	0.60 ± 0.01	0.61 ± 0.08	0.98	1.34	TB9Cs5C2	2.24
LSUα	Cm1006	0.60 ± 0.01	0.77 ± 0.06	0.78	1.27	TB9Cs5C1	−0.48
LSUα	Am1024	0.75 ± 0.01	0.86 ± 0.04	0.87	1.44	TB11Cs1C3	1.43
LSUα	Gm1028	0.50 ± 0.02	0.72 ± 0.08	0.70	1.14	TB9Cs2C5	−0.33
LSUα	Um1145	0.58 ± 0.04	0.84 ± 0.00	0.69	0.99	TB10Cs3C4	−0.70
LSUα	Am1180	0.01 ± 0.00	0.77 ± 0.05	0.01	1.01	TB9Cs5C1	−0.48
LSUα	Um1181	0.84 ± 0.02	0.94 ± 0.02	0.90	0.97	TB10Cs3C4	−0.70
LSUα	Gm1267	0.54 ± 0.04	0.83 ± 0.03	0.65	1.31	TB9Cs4C1	−0.83
LSUα	Um1448	0.49 ± 0.01	0.69 ± 0.05	0.71	1.08	TB8Cs2C2	0.67
LSUα	Gm1605	0.62 ± 0.02	0.79 ± 0.06	0.79	1.52	TB10Cs2”C3	1.03
LSUα	Cm1608	0.53 ± 0.01	0.74 ± 0.07	0.72	1.24	TB8Cs3C1	0.83
LSUα	Am1620	0.76 ± 0.02	0.77 ± 0.05	0.99	1.12	TB8Cs3C1	0.83
LSUα	Gm1621	0.50 ± 0.01	0.26 ± 0.07	1.94	0.99	TB8Cs3C1	0.83
LSUα	Am1665	0.64 ± 0.02	0.73 ± 0.01	0.88	0.98	TB6Cs1ʹC1	0.02
LSUα	Gm1709	0.72 ± 0.01	0.83 ± 0.05	0.87	0.76	TB8Cs1C1	1.06
LSUα	Um1742	0.80 ± 0.01	0.84 ± 0.03	0.96	1.02	TB7Cs2C1	−0.34
5.8 S	Am41	0.80 ± 0.01	0.82 ± 0.05	0.97	1.22	TB6Cs1ʹC1	0.02
5.8 S	Am43	0.64 ± 0.00	0.56 ± 0.06	1.14	1.44	TB6Cs1ʹC1	0.02
5.8 S	Gm75	0.55 ± 0.01	0.64 ± 0.08	0.85	1.42	TB8Cs3C3	0.15
5.8 S	Am163	0.95 ± 0.00	0.94 ± 0.02	1.01	1.15	TB9Cs4C2	0.70
5.8 S	Um167	0.88 ± 0.00	0.92 ± 0.01	0.96	1.34	TB9Cs4C2	0.70