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. 2020 May 31;17(9):1331–1341. doi: 10.1080/15476286.2020.1762054

Figure 3.

Figure 3.

Gel-shift analysis of RNA structural motifs with RBS1. (A) Schematic representing the H12 probes used in the assay (top). (Bottom) Graph representing the adjusted curves obtained from the quantification (mean ± SD) of three independent gel-shift assays using H12, SL1, and SL2 probes incubated with increasing amounts of His-RBS1 protein. (B) Representative examples of the gel-shift assays conducted with labelled H12, SL1 or SL2 RNAs. (C) Gel-shift assays conducted with labelled RNA2 (encompassing nucleotides 4204-4259 of Gemin5 mRNA), and RNA3 (UUUCCUUU synthetic RNA).