Figure 5.

RBS1 substitution mutants display lower RNA-binding capacity. (A) Schematic depicting the substitution His-RBS1 proteins used in gel-shift analysis. (B) Graph representing the adjusted curves obtained from the quantification (mean ± SD) of independent assays using H12 or SL1 probes incubated with increasing amounts of RBS1-WT (black line), P-E (blue line) or SS-DD (red line) proteins. Representative examples of the gel-shift assays conducted with labelled H12 or SL1 with SS-DD or P-E proteins (right panels).