Figure 6.

RBS1 substitution mutants lose the stimulatory effect on H12-dependent translation. (A) Schematic representing the proteins and RNA reporters expressed in HEK293 cells. (B) Expression of the TAP fusion peptide (Ctrl), RBS1-WT, SS-DD, and P-E proteins were immunodetected with anti-CBP. Tubulin was used as loading control. (C) Normalized luciferase activity obtained for luc-H12 mRNA relative to cap-luc transcript in cells expressing Ctrl, RBS1-WT, SS-DD, or P-E proteins. Values represent the mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test).