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. 2020 Mar 15;17(6):784–793. doi: 10.1080/15476286.2020.1726637

Figure 3.

Figure 3.

LncRNA MAGI2-AS3 inhibits LSCs self-renewal by elevating LRIG1 expression. A: Analysis of the GSE17054 dataset revealed the positive correlation between MAGI2-AS3 and LRIG1; B: LRIG1 mRNA expression in LSCs and normal human haematopoietic stem cells determined by RT-qPCR; C: LRIG1 protein expression in LSCs and normal human haematopoietic stem cells determined by western blot analysis; D: LRIG1 mRNA expression in LSCs with MAGI2-AS3 over-expression revealed by RT-qPCR; E: LRIG1 protein expression in LSCs with MAGI2-AS3 over-expression shown by western blot analysis; F: LRIG1 protein expression in LSCs following LRIG1 over-expression shown by western blot analysis; G: Sphere-forming ability of LSCs examined by sphere formation assay (× 200); H: Colony formation ability of LSCs using colony formation assay; I: LRIG1 mRNA expression in cells with oe-MAGI2-AS3 and sh-LRIG1 determined by RT-qPCR; J: LRIG1 protein expression in cells with oe-MAGI2-AS3 and sh-LRIG1 detected by western blot analysis; K: Sphere-forming ability of LSCs using sphere formation assay (× 200); L: Colony formation ability of LSCs via colony formation assay; * p < 0.05 vs. normal human haematopoietic stem cells; # p < 0.05 vs. the oe-NC group; & p < 0.05 vs. the oe-MAGI2-AS3 + sh-NC group. Data were expressed as mean ± standard error and analysed using unpaired t-test.